Monoclonal antibodies for the detection of pyrethroids and a process for their production

US5723306A · kind A · utility

Assignee

• DRAGERWERK AKTIEGESELLSCHAFT · DE

Inventors

• Sabine Pullen · Freising, DE
• Berthold Hock · Freising, DE
• Thomas Wuske · Malente, DE
• Andreas Manns · Lübeck, DE

Key dates

• Filing date: Aug 24, 1994
• Grant date: Mar 3, 1998
• Expiry date: Aug 24, 2014

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N33/5308
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Highly selective monoclonal antibodies for pyrethrins and pyrethroids which, in their function as pesticides, contain a phenoxybenzyl and/or a cyclopropane component. The number of pyrethroids which can be selectively detected is increased and the possibility of differentiating among the various pyrethrins, pyrethroids and their metabolites is possible. An easy-to-use test kit detects pyrethrins and pyrethroids. A conjugate of a metabolite of the pyrethrins/pyrethroids (especially permethric acid or phenoxybenzoic acid) is coupled as hapten to a carrier protein to obtain the immunogen, which is used for immunization and for the subsequent cloning of suitable hybridomas.