Apparatus and method for electrostatic endothelial cell seeding and DNA transfection in a vascular prosthesis
US5723324A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Sep 30, 1996 |
| Grant date | Mar 3, 1998 |
| Priority date | — |
| Expiry date | Sep 30, 2016 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S623/92
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
An apparatus and method are provided for in vitro seeding endothelial cells and transfecting DNA on the luminal wall of a vascular prosthesis. The apparatus has an internal conductor and an external conductor, and a support means which maintains a suspension containing at least one of endothelial cells and DNA plasmids in contact with the luminal wall and which maintains the luminal wall between the internal and external conductors while alteration of an electrical charge transfers at least one of endothelial cells and DNA plasmids from the suspension to the luminal wall. The internal conductor is an insulated wire and the external conductor has a bore through which the internal conductor passes. A vascular prosthesis containing a suspension of endothelial cells and/or DNA plasmids sealed within its lumen fits within the bore of the external conductor and the internal conductor wire passes through the lumen. While the prosthesis is rotated, an electrical potential is applied between the internal and external conductors altering the surface charge of the luminal wall of the prosthesis to facilitate adhesion of the endothelial cells and/or DNA plasmids to the lumen wall. When both en…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.