Genetically engineered reporter bacteria for the detection of bacteriophage
US5723330A · kind A · utility
18Cited by
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3Claims
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Key dates
| Filing date | Dec 19, 1995 |
| Grant date | Mar 3, 1998 |
| Priority date | — |
| Expiry date | Dec 19, 2015 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/66
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for testing for target bacteria involves adding bacteriophage to a sample to infect the bacteria in the sample; killing extracellular bacteriophage without killing phage-infected bacteria; amplifying bacteriophage remaining in the sample; and causing the bacteriophage to infect reporter bacteria and thereby produce an observable signal. The reporter bacteria are genetically engineered to have an indicator gene which on expression gives rise to a detectable signal, wherein expression of the indicator gene is initiated on bacteriophage infection of the bacteria.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.