Test media and quantitative method for identification and differentiation of biological materials in a test sample
US5726031A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Mar 26, 1996 |
| Grant date | Mar 10, 1998 |
| Priority date | — |
| Expiry date | Mar 26, 2016 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/879
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A test method and medium for quantitatively identifying and distinguishing biological materials in a test sample. A first biological material has enzyme specificity for a first chromogenic substrate, a second biological material has enzyme specificity for a second chromogenic substrate, and a third biological material has specificity for one of the substrates. The chromogenic substrates form respective first and second colored water insoluble compounds upon reaction with specific enzymes. The first and second biological materials are capable of fermenting a sugar, and the third material does not ferment sugar. The test medium is adjusted to a pH conducive for color change of a pH indicator upon acidification due to fermentation, resulting in the formation of a zone of a third color around the water insoluble compounds of the sugar-fermenting materials. The sample is incubated, and examined for the presence of colonies of the first biological material, having the first color and an encircling zone of the third color; for colonies of the second material, having the second color and the encircling colored zone; for colonies of a third material having the first color, and not having th…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.