Vectors and transformed host cells for recombinant protein production at reduced temperatures

US5726039A · kind A · utility

Assignee

• YISSUM RESEARCH DEVELOPMENT COMPANY OF THE HEBREW UNIVERSITY OF JERUSALEM LTD. · IL

Inventors

• Amos B. Oppenheim · Jerusalem, IL
• Hilla Giladi · Mevaseret Tsiyon, IL
• Daniel Goldenberg
• Simi Kobi · Jerusalem, IL
• Idit Azar · Rehovot, IL

Key dates

• Filing date: Jul 20, 1995
• Grant date: Mar 10, 1998
• Expiry date: Jul 20, 2015

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N15/70
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The invention relates to an expression vector, the nucleic acid sequence of which comprises a promoter that is capable of controlling, when the vector is in a bacterial host and the temperature is lowered to below about 20.degree. C., the production of recombinant peptide or protein encoded by a gene contained within the vector. The invention also relates to host cells containing the vectors and to a method of producing a recombinant protein.