Denaturing separation matrix having hydroxyethyl cellulose for nucleic acid electrophoresis
US5728282A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Apr 12, 1996 |
| Grant date | Mar 17, 1998 |
| Priority date | — |
| Expiry date | Apr 12, 2016 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N27/44747
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A capillary electrophoresis separation matrix for single-stranded nucleic acids, along with methods for using and preparing the matrix, are disclosed. The separation matrix provides denaturing conditions and contains hydroxyethyl cellulose (HEC) in combination with urea, and preferably also includes formamide. The separation matrix may be used for DNA sizing and sequencing applications and provides a single-base resolution to approximately 500 base pairs. The separation matrix is inexpensive, easy to prepare, requires no polymerization steps, and is of low enough viscosity to be pumped easily into and out of capillary tubes for electrophoresis. The low viscosity allows for high throughput of samples and reuse of the capillary tubes for numerous separations.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.