Clostripain catalyzed hydrolysis of preproinsulin analogs into corresponding insulins

US5728543A · kind A · utility

Assignee

• Hoechst GmbH · DE

Inventors

• Michael Dorschug · Bochum, DE
• Klaus-Peter Koller · Bad Soden, DE
• Rudiger Marquardt · Frankfurt am Main, DE
• Johannes Meiwes · Idstein, DE

Key dates

• Filing date: Aug 8, 1994
• Grant date: Mar 17, 1998
• Expiry date: Aug 8, 2014

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K14/62
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A process is described to specifically hydrolyze the amino acid chain of preproinsulin analogs to give the corresponding insulins. The hydrolysis is catalyzed by clostripain and, if necessary, carboxypeptidase B.