Preparation of d-amino acids by direct fermentative means
US5728555A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Sep 30, 1996 |
| Grant date | Mar 17, 1998 |
| Priority date | — |
| Expiry date | Sep 30, 2016 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12P13/22
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to materials and methods for production of natural and unnatural D-amino acids. In particular, the present invention relates to a fermentation method for the production of D-amino acids using recombinant host cells. Specifically, the invention relates to a method for producing a D-amino acid in a cell, comprising: PA1 (a) incorporating into the cell a D-aminotransferase gene and a L-aminodeaminase gene; PA1 (b) culturing the cell in a cell culture medium; and PA1 (c) isolating the D-amino acid from the cell culture medium. The invention also relates to a method for producing D-phenylalanine in a cell, comprising: PA1 (a) incorporating into the cell a D-aminotransferase gene, a L-aminodeaminase gene and means for increasing production of phenylpyruvate; PA1 (b) culturing the cell in a cell culture medium; and PA1 (c) isolating the D-phenylalanine from the cell culture medium. The invention also relates to the preparation of recombinant cells for use in the production of enantiomerically pure D-amino acids.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.