Monitoring method of stain solution for particle analysis and calibration method of particle analysis
US5728582A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Apr 17, 1995 |
| Grant date | Mar 17, 1998 |
| Priority date | — |
| Expiry date | Apr 17, 2015 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/101666
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Sample fluids including biological particles are mixed with a stain solution, and stained biological particles are fed into a flow through cell. Still frame images of the particles flowing through the flow-through cell are photographed by a television camera, and are processed for particle classification. Prior to such processing of biological particle images, a calibration coefficient is calculated using reference particles. The reference particles may include polystyrene resin which contains functional radicals such as sulfonic radicals, and are stained by mixing the suspended fluid containing these reference particles with the stain solution. The calibration coefficient is calculated according to the information on the stain in the still frame images, and is used to calibrate biological particle data.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.