Chimaeric gene coding for a transit peptide and a heterologous polypeptide

US5728925A · kind A · utility

Assignees

• Plant Genetic Systems N.V. · BE
• Bayer A.G. · DE

Inventors

• Luis Rafael Herrera-Estrella · Evergem, BE
• Guido Van Den Broeck · Evergem, BE
• Marc Van Montagu · Brussels, BE
• Peter Schreier · Cologne, DE
• Jeff Schell · Cologne, DE
• Hans J. Bohnert · Tucson, US
• Anthony R. Cashmore · New Rochelle, US
• Michael Paul Timko · Charlottesville, US
• Albert P. Kausch · Stonington, US

Key dates

• Filing date: Apr 28, 1995
• Grant date: Mar 17, 1998
• Expiry date: Apr 28, 2015

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N15/8221
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Chimaeric DNA sequence which encodes: 1) a transit peptide of a cytoplasmic precursor of a chloroplast protein or polypeptide of a plant and 2) a protein or polypeptide that is heterologous to the transit peptide. The chimaeric DNA sequence can be used as a vector for transforming a plant cell so that a chimaeric precursor of the heterologous protein or polypeptide is produced in the cytoplasm of the cell and the chimaeric precursor then transports the heterologous protein or polypeptide in vivo into a chloroplast of the cell.