Compositions and methods for detecting target nucleic acid sequences utilizing adjacent sequence-enzyme molecules
US5731146A · kind A · utility
61Cited by
5References
21Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jun 6, 1995 |
| Grant date | Mar 24, 1998 |
| Priority date | — |
| Expiry date | Jun 6, 2015 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6823
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Briefly stated, the present invention provides novel compositions and methods for detecting target nucleic acid sequences utilizing adjacent sequence-enzyme molecules. Within one aspect of the present invention, oligonucleotide-enzyme fusion molecules are provided, comprising an enzyme capable of cleaving scissile linkages and an oligonucleotide having the structure (NA.sub.1).sub.x --S.sub.z --(NA.sub.2).sub.y wherein NA.sub.1 and NA.sub.2 are nucleic acid sequences, S is a scissile nucleic acid linkage, x, y and z are integers from 1 to 100 and n is an integer from 1 to 10.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.