Antibiotic cryptdin peptides and methods of their use

US5731149A · kind A · utility

Assignee

• The Shriner's Hospital For Crippled Children · US

Inventors

• Michael E. Selsted · Los Angeles, US
• Andre J. Ouellette · Lynn, US

Key dates

• Filing date: Jun 7, 1995
• Grant date: Mar 24, 1998
• Expiry date: Jun 7, 2015

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2333/4721
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The present invention provides substantially purified cryptdin peptides having a consensus amino acid sequence: EQU X.sub.1 --C--X.sub.2 --C--R--X.sub.3 --C--X.sub.4 --E--X.sub.5 --C--X.sub.6 --C--C--X.sub.7 wherein PA1 X.sub.1 is 3 to 9 amino acids; PA1 X.sub.2 is one amino acid, preferably Y, H or R; PA1 X.sub.3 is 2 or 3 amino acids; PA1 X.sub.4 is three amino acids; PA1 X.sub.5 is five amino acids; PA1 X.sub.6 is 6 to 10 amino acids; and PA1 X.sub.7 is 0 to 9 amino acids. The invention also provides a substantially purified mouse cryptdin having a consensus amino acid sequence: EQU X.sub.1 --L--X.sub.2 --C--Y--C--R--X.sub.3 --C--K--X.sub.4 --E--X.sub.5 --G--T--C--X.sub.6 --C--C--X.sub.7 wherein PA1 X.sub.1 is 3 or 4 amino acids, preferably LRD, LSKK (SEQ ID NO: 1) or LRG; PA1 X.sub.2 is 1 amino acid, preferably V, L or I; PA1 X.sub.3 is 3 amino acids, preferably KGH or *RG, where * is S, T, K, I or A; PA1 X.sub.4 is 2 amino acids, preferably GR, RR or RG; PA1 X.sub.5 is 3 amino acids, preferably RMN, RVR, RVF HMN or HIN; PA1 X.sub.6 is 6 to 9 amino acids, preferably GIRFLY (SEQ ID NO: 2) or RNLFLTFVF (SEQ ID NO: 3), RRGHLMYTL (SEQ ID NO: 4) or RKGHL*YT* (SEQ ID NO: 5), where * …