Bacillus-derived transglutaminase
US5731183A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Feb 9, 1996 |
| Grant date | Mar 24, 1998 |
| Priority date | — |
| Expiry date | Feb 9, 2016 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/839
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to (1) a transglutaminase (hereinafter referred to as TG) isolated from a Bacilli such as those of Bacillus subtilis, (2) a fraction having transglutaminase activity, and (3) a method for producing a protein, a non-proteinaceous amino acid polymer, a peptide or derivatives thereof having a crosslinked structure, by crosslinking the glutamine and lysine residues in the same with the TG or the fraction having TG activity to thereby form intermolecular or intramolecular, crosslinked .epsilon.-(.gamma.-Glu)-Lys bonds. The present invention also relates to (4) a DNA coding for a TG derived from a Bacilli such as Bacillus subtilis, (5) a vector comprising said DNA coding for the TG, (6) a cell transformed with the vector, and (7) a method for producing a Bacillus-derived transglutaminase by incubating the transformant. The crosslinked polymers produced using the Bacillus-derived TG of the present invention can be used in foods such as tofu (soybean curd), pudding, yogurt, cheese, ground fish meat, boiled fish paste, sausage and other fish and meat products and also in cosmetics, etc.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.