Methods for producing members of specific binding pairs
US5733743A · kind A · utility
Assignees
Inventors
Key dates
| Filing date | Nov 18, 1994 |
| Grant date | Mar 31, 1998 |
| Priority date | — |
| Expiry date | Nov 18, 2014 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2319/735
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods, recombinant host cells and kits are disclosed for the production of members of specific binding pairs (sbp), e.g. antibodies, using display on the surface of secreted replicable genetic display packages (rgdps), e.g. filamentous phage. To produce a library of great diversity, recombination occurs between first and second vectors comprising nucleic acid encoding first and second polypeptide chains of sbp members respectively, thereby producing recombinant vectors each encoding both a first and a second polypeptide chain component of an sbp member. The recombination may take place in vitro or intracellularly and may be site-specific, e.g. involving use of the loxP sequence and mutants thereof. Recombination may take place after prior screening or selecting for rgdps displaying sbp members which bind complementary sbp member of interest.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.