Enzyme and its use in preparing (S)-pipecolic acid
US5733754A · kind A · utility
1Cited by
2References
11Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Apr 8, 1996 |
| Grant date | Mar 31, 1998 |
| Priority date | — |
| Expiry date | Apr 8, 2016 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12R2001/05
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
An enzyme is provided which has acylase activity capable of hydrolyzing N-acetyl-(R,S)-pipecolic acid stereoselectively to give (S)-pipecolic acid, wherein this activity is greater than that on N-acetyl-S-proline, at pH 7.5, 25.degree. C., and substrate concentration 20 g/l, in 75 mM Tris buffer. The enzyme is obtainable from the species of Alcaligenes denitrificans deposited as NCIMB 40587. A microorganism having therein the enzyme also is provided, as is a process for preparing (S)-pipecolic acid comprising contacting a mixture of enantiomers of an N-acyl-pipe colic acid with the enzyme or microorganism.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.