Methods and compositions for high protein production from non-native DNA
US5733782A · kind A · utility
11Cited by
8References
24Claims
0Family size
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Key dates
| Filing date | Jun 5, 1995 |
| Grant date | Mar 31, 1998 |
| Priority date | — |
| Expiry date | Jun 5, 2015 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S530/867
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Disclosed herein are improved methods and compositions for achieving enhanced protein production expressed from non-native gene constructs, including single chain sFv and derivative sequences. The methods and compositions are particularly useful for creating stably transfected, constitutively expressing immortalized mammalian cell lines that exhibit high recombinant protein productivity while maintaining a low copy number per cell of the non-native recombinant DNA sequence encoding the protein of interest.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.