Patent · US Expired

Method for detection of contaminants

US5736351A · kind A · utility

19Cited by
10References
24Claims
0Family size

Assignee

Inventors

Key dates

Filing dateJan 15, 1997
Grant dateApr 7, 1998
Priority date
Expiry dateJan 15, 2017

Classification

  • Technology area (CPC Y)Emerging Cross-Sectional Technologies
  • CPC primaryY10S435/968
  • WIPO fieldMeasurement
  • WIPO sectorInstruments

Abstract

A method and device for determining the presence and concentration of total microbial contamination or the presence and concentration of a specific microbial species on a surface is described. The method consists of a means of a collection device and fluid for removing the microbes from the surface and suspending them in a fluid phase. An aliquot of the fluid phase is introduced into a disposable test ticket which allows filtration of the sample to remove extraneous substances including somatic cells, and concentration of the microbes. The total concentration of microbes is determined by adding a somatic and bacterial releasing reagent to a disposable test device which comprises a membrane containing the luminescent reagents luciferin and luciferase, and introducing the disposable test device into a luminometer that can read the luminescence from the underside.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.