Method of measuring enzyme reaction by Raman scattering
US5741660A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jan 30, 1996 |
| Grant date | Apr 21, 1998 |
| Priority date | — |
| Expiry date | Jan 30, 2016 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2021/651
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
As to reaction solutions in which the amount of each potassium ferricyanate, glucose oxidase and glucose substrate are set at a constant value while glucose concentrations are varied, intensity changes of peaks at a shift wavenumber of 2081 cm.sup.-1 in light scattering spectra are measured every 10 seconds, the maximum values of the intensity changes of the peaks at the respective glucose concentrations obtained, for obtaining a calibration curve from correlations between the maximum values of the reaction velocities and the glucose concentrations. The calibration curve is employed for carrying out quantitative measurement of an unknown sample.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.