In vitro packaging of adeno-associated virus DNA
US5741683A · kind A · utility
105Cited by
2References
11Claims
0Family size
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Key dates
| Filing date | Jun 7, 1995 |
| Grant date | Apr 21, 1998 |
| Priority date | — |
| Expiry date | Jun 7, 2015 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2750/14152
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
A method for in vitro packaging of adeno-associated viral particles is described. The procedure involves the preparation of cell-free extracts containing all the essential components for packaging. Homogeneous purified substrate DNA for packaging may be prepared separately. The in vitro packaged AAV particles are useful in transduction of mammalian cells and for gene therapy in animals. In one described method, the DNA packaged into AAV particles is not limited by the size constraints characteristic of in vivo packaged AAV particles.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.