Preparation of pharmaceutical grade hemoglobins by heat treatment in partially oxygenated form
US5741894A · kind A · utility
7Cited by
45References
21Claims
0Family size
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Key dates
| Filing date | Sep 22, 1995 |
| Grant date | Apr 21, 1998 |
| Priority date | — |
| Expiry date | Sep 22, 2015 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA61K38/00
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
During the purification of pharmaceutical grade crosslinked hemoglobin mixtures of crosslinked and uncrosslinked hemoglobin are heated in the presence of nonstoichiometric amounts of oxygen, resulting in the selective precipitation of the uncrosslinked hemoglobin. After separation of the precipitated uncrosslinked tetramers, the crosslinked hemoglobin remaining in the supernatant is so purified that a further chromatography purification step is unnecessary. This hemoglobin is highly crosslinked absolutely free of chromatography fines, and has a low methemoglobin content.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.