Human parainfluenza virus-1 assay
US5744299A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Nov 3, 1995 |
| Grant date | Apr 28, 1998 |
| Priority date | — |
| Expiry date | Nov 3, 2015 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6816
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention is a method for evaluating a biological sample for the presence or absence of human parainfluenza virus 1 (HPIV-1) infection and for the quantitation of HPIV-1. This method comprises the steps of isolating RNA from a biological sample, creating cDNA from the isolated RNA, exposing the cDNA to a pair of oligonucleotide primers selected from sequences from the group consisting of SEQ ID NOs:1-9 and inverse complements of SEQ ID NOs:1-9 under conditions in the which the primers will amplify a human parainfluenza-1 sequence if the human parainfluenza-1 virus is present, and determining whether the amplified sequence is present by EHA. The present invention is also a kit for the detection and quantitation of parainfluenza virus 1 and a kit for the detection of parainfluenza virus-1, 2 and 3 genomic RNA.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.