Periplasmic membrane-bound system for detecting protein-protein interactions
US5744314A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | May 3, 1996 |
| Grant date | Apr 28, 1998 |
| Priority date | — |
| Expiry date | May 3, 2016 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2740/16222
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to: PA1 (1) a fusion protein having a dimerizing domain with or without a ligand-binding region and toxR DNA-binding and hydrophobic transmembrane regions; PA1 (2) host cells comprising the fusion protein and a nucleic acid molecule having a reporter gene operatively linked to the ctx operon, wherein dimerization (ligand-dependent or -independent) is signaled by expression of the reporter gene; PA1 (3) a nucleic acid molecule coding for the fusion protein; PA1 (4) an expression vector comprising a coding region for the fusion protein; PA1 (5) a process for detecting dimer formation (ligand dependent or ligand independent) of the fusion protein, which comprises treating a culture of the host cells with a ligand, ligand mimetic, or dimerization inhibitor, and screening for expression of the reporter gene. The present invention can be used to generate a signal from a variety of ligand-binding domains, allowing ligand binding to be indicated by a simple colorimeteric test or antibiotic resistance. The fusion proteins could include therapeutically relevant domains, so that biologically pertinent interactions can be indicated by a readily measurable signal.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.