Expansion of human hematopoietic progenitor cells in a liquid medium
US5744361A · kind A · utility
51Cited by
15References
7Claims
0Family size
Assignee
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Key dates
| Filing date | Jun 6, 1995 |
| Grant date | Apr 28, 1998 |
| Priority date | — |
| Expiry date | Jun 6, 2015 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2501/23
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The use of individual or combinations of cytokines, particularly IL-3, GM-CSF, and c-kit ligand are employed for long-term hematopoiesis in serum free culture in the absence of stromal cells. The cultures can be used for evaluating compounds and their effect on hematopoiesis, particularly as to lifetime and nature of differentiation. In addition, the expanded cells may be used for engraftment in a mammalian host or enhancement of particular cell lineages in a mammalian host. The subject systems may be used with any mammalian hemopoietic cells, but finds particular application with primates, more particularly humans.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.