Method of making calcification-resistant bioprosthetic tissue

US5746775A · kind A · utility

Assignee

• The Board of Regents of the University of Michigan · US

Inventors

• Robert J. Levy · Yeadon, US
• Danielle Hirsch · Jerusalem, IL

Key dates

• Filing date: Oct 21, 1993
• Grant date: May 5, 1998
• Expiry date: Oct 21, 2013

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S623/922
• WIPO field: Medical technology
• WIPO sector: Instruments

Abstract

A method of treating a collagenous biomaterial, such as porcine aortic valve leaflets or bovine pericardium, by exposing the biomaterial to an alcohol to inhibit in vivo calcification. The biomaterial, preferably glutaraldehyde-pretreated, is subjected to an aqueous solution of 60% to 80% lower aliphatic alcohol, such as ethanol for a period of at least 20 minutes, and preferably, 24 to 72 hours. The biomaterial is rinsed, and then stored in either a glutaraldehyde-free environment or an ethanolic solution of glutaraldehyde. In some embodiments, the treatment solutions include an additional anticalcification agent which may be a soluble salt of a metallic cation, such as Al.sup.+3 or Fe.sup.+3.