Epitope mapping of the c33 region of HCV
US5747240A · kind A · utility
1Cited by
1References
10Claims
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Inventors
Key dates
| Filing date | Mar 28, 1995 |
| Grant date | May 5, 1998 |
| Priority date | — |
| Expiry date | Mar 28, 2015 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2770/24222
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
An assay for detection of serum antibodies having specificity to hepatitis C viral antigens utilizes the carboxy-terminal 102 amino acids of the c33c region of nonstructural gene 3 of HCV containing substantially all the epitopes of the gene 3 protein. In the preferred embodiment the recombinant 102 amino acid peptide is coated onto a solid support, and incubated in the presence of serum obtained from a patient infected with HCV. The presence of antibodies bound to the antigen coated support is detected in a sandwich format utilizing a second reporter-conjugated antibody.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.