Genetic markers and methods for the detection of escherichia coli serotype-0157:H7
US5747257A · kind A · utility
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4References
7Claims
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Key dates
| Filing date | Feb 29, 1996 |
| Grant date | May 5, 1998 |
| Priority date | — |
| Expiry date | Feb 29, 2016 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/689
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method, diagnostic sequences and primers are provided that are useful in the identification of the Escherichia coli 0157:H7 serotype. The method first involves the identification of a RAPD-amplified DNA fragment common to 0157:H7 Escherichia coli, the identification of the most conserved regions of that fragment, and the preparation of specific primers useful for detecting the presence of a marker within the fragment whereby that set of primers is then useful in the identification of all 0157:H7 Escherichia coli.
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