Method for measuring the amount of a cell-associated molecule
US5747265A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | May 14, 1993 |
| Grant date | May 5, 1998 |
| Priority date | — |
| Expiry date | May 14, 2013 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/6863
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The invention is directed to methods and a device for detecting or measuring the amount of a cell-associated molecule in a biological fluid sample. Cell-associated molecules which can be detected or measured include, but are not limited to, cell surface antigens, intracellular cytokines, microbial antigens, pharmacological agents or their metabolites, and nucleic acids. In the preferred embodiment, a sample of whole blood is collected on filter paper and dried. A dot is punched form the dried blood spot and the analyte of interest eluted from the paper with a buffered, relatively highly concentrated detergent solution. The eluate is then assayed in a standard assay, such as an immunoassay.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.