D-sorbitol dehydrogenase
US5747301A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Feb 26, 1996 |
| Grant date | May 5, 1998 |
| Priority date | — |
| Expiry date | Feb 26, 2016 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/823
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A D-sorbitol dehydrogenase in homogenous form, which catalyzes the oxidation of D-sorbitol to L-sorbose, has a molecular structure consisting of homologous subunits of molecular weight 79,000.+-.5,000 each, a substrate specificity for polyols and an optimum pH of 6.0-7.0. Said D-sorbitol dehydrogenase is producible by a process comprising cultivating a microorganism belonging to the genus Gluconobacter or Acetobacter, or a mutant or variant thereof, which is capable of producing the D-sorbitol dehydrogenase in the cells and isolating it from the cells, e.g. by disrupting the cells and isolation from cell-free extract of the disrupted cells. The so-isolated D-sorbitol dehydrogenase is useful for catalyzing the oxidation of D-sorbitol to L-sorbose, the latter being an important intermediate for the production of vitamin C.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.