Process for the depletion or removal of endotoxins

US5747663A · kind A · utility

Assignee

• QIAGEN GmbH · DE

Inventors

• Metin Colpan · Essen, DE
• Peter Moritz · Wiesendangen, CH
• Joachim Schorr · Düsseldorf, DE

Key dates

• Filing date: Sep 30, 1996
• Grant date: May 5, 1998
• Expiry date: Sep 30, 2016

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10T436/10
• WIPO field: Chemical engineering
• WIPO sector: Chemistry

Abstract

A process is disclosed for reducing or removing endotoxins from compositions containing therapeutic active substances extracted from natural sources by genetic engineering and/or biotechnology. For that purpose, the compositions are treated with chromatographic materials. The natural sources are disintegrated, the thus obtained fractions are, if required, centrifuged, filtered or treated using affinity chromatography methods, the fractions are pre-incubated in an aqueous salt solution and detergents, are treated with anion exchange materials, then washed with another salt solution. The active substances are eluted from the anion exchanger then further purified in a manner known per se.