Host organism capture
US5750346A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Aug 7, 1996 |
| Grant date | May 12, 1998 |
| Priority date | — |
| Expiry date | Aug 7, 2016 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/70
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Cloning systems useful for the isolation of recombinant nucleic acid are disclosed in which the recombination of cloning-system nucleic acid and foreign nucleic acid is linked to the expression of a moiety on the surface of a host organism, the moiety being a first member of a binding pair. When recombination occurs between the nucleic acid and the foreign nucleic acid, the moiety is expressed on the surface of the host organism. The isolation of recombinant nucleic acid is then performed by attaching a second member of the binding pair to a solid support and contacting the host organism with the support. When the first member of the binding pair is expressed on the surface of the host organism, the host organism binds to the second member of the binding pair attached to the solid support, thereby selectively isolating those organisms. Other aspects of the invention include devices for the isolation of individual cells that differentially express binding moieties on their surface. The devices may be adapted for polynucleotide sequencing and analysis of polynucleotides in the isolated cells. Additional aspects of the invention include methods of using the devices for single cell iso…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.