Purified saccharose-synthase, process for its production and its use

US5750389A · kind A · utility

Assignee

• Forschungszentrum Jülich GmbH · DE

Inventors

• Lothar Elling · Aachen, DE
• Maria-Regina Kula · Jülich, DE

Key dates

• Filing date: Jan 6, 1995
• Grant date: May 12, 1998
• Expiry date: Jan 6, 2015

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12P19/38
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Nucleotide sugars, especially UDP, ADP, CDP or TDP saccharoses can be enzymatically obtained by the reaction of nucleoside diphosphates with di or trisaccharides with a saccharose synthase in which the virtual absence of nucleoside phosphatases (0.1% or less) can be ensured by special purification methods and sensitive detection. The purification of the raw extract, obtained preferably from rice grains, comprises especially the application of the ultra-filtered extract containing 50 mM KCl with a pH 8 on a sepharose Q column and a gradient elution out of the column at a pH 8 with 50 to 500 mM KCl.