Reversible nuclear genetic system for male sterility in transgenic plants
US5750868A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Dec 8, 1994 |
| Grant date | May 12, 1998 |
| Priority date | — |
| Expiry date | Dec 8, 2014 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S47/01
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Plant development can be altered by transforming a plant with a genetic construct that includes regulatory elements and structural genes capable of acting in a cascading fashion to produce a reversible effect on a plant phenotype. A first genetic construct comprising (i) an operator that is capable of controlling expression of a dominant negative gene, (ii) a dominant negative gene that, when expressed in a plant disrupts pollen formation or function, (iii) a tissue specific promoter that regulates the expression of a gene encoding a DNA-binding protein which binds to the operator and activates transcription, and (iv) a gene encoding a DNA binding region and an activating domain, causes male sterility in a plant. In particular, the present invention relates to the use of a DAM-methylase gene as a dominant negative gene and an anther-specific promoter. Male sterility is reversed by incorporation into a plant of a second genetic construct which represses the dominant negative gene.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.