Cysteine protease gene defective baculovirus, process for its production, and process for the production of economic protein by using the same
US5753220A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Mar 15, 1995 |
| Grant date | May 19, 1998 |
| Priority date | — |
| Expiry date | Mar 15, 2015 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2710/14143
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
For the expression of a foreign gene, a virus is provided in which at least a part of a cysteine protease gene present on a genome of a baculovirus has been deleted or substituted by a marker gene. A process is also disclosed for the formation of the virus. A transfer vector for the deletion of a cysteine protease gene is also described. This transfer vector comprises a DNA fragment of a nuclear polyhedrosis virus genome, in which at least a part of a cysteine protease gene has been substituted by a marker gene, and a plasmid vector fragment. Also disclosed is a process for the production of an economic protein. This production process comprises inoculating a host insect with a gene recombination virus, said gene recombination virus having been obtained by deleting or substituting by a marker gene at least a part of a cysteine protease gene present on a genome of a baculovirus or a nuclear polyhedrosis virus; expressing an economic protein gene inserted downstream a polyhedral gene promoter, granulin gene promoter or p10 gene promoter of the gene recombination virus; and collecting the economic protein.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.