Method for the sensitive detection of nucleic acids

US5753433A · kind A · utility

Assignee

• Boehringer Mannheim GmbH · DE

Inventors

• Christoph Kessler · Munich, DE
• Rudiger Ruger · Tutzing, DE
• Rudolf Seibl · Penzberg, DE
• Cornelia Kruse-Müller · Tutzing, DE
• Sibylle Berner · Starnberg, DE

Key dates

• Filing date: Sep 19, 1994
• Grant date: May 19, 1998
• Expiry date: Sep 19, 2014

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6855
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Method for the specific detection of nucleic acids in a sample by reaction of the sample with one or several labelled nucleotide triphosphates and one or several enzymes which catalyse the production of a labelled nucleic acid B which contains this nucleotide, non-thermally denaturation, reacting the sample with a nucleic acid probe C which is sufficiently complementary to nucleic acid B and which contains at least one immobilizable group, if desired, contacting the nucleic acid hybrid D formed with a solid phase which recognizes and binds the immobilizable group, removing the liquid from the solid phase and determining the label on the solid phase as a measure for the presence of the nucleic acid.