Secretion leader trap cloning method
US5753462A · kind A · utility
8Cited by
1References
22Claims
0Family size
Assignee
Inventor
Key dates
| Filing date | Feb 6, 1997 |
| Grant date | May 19, 1998 |
| Priority date | — |
| Expiry date | Feb 6, 2017 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2319/90
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Expression vectors and methods for cloning amino-terminal signal sequences are disclosed. These vectors and methods are useful tools for cloning, identifying and isolating previously unknown signal sequences. In turn, an orphan signal sequence can be used to clone, identify and isolate the corresponding orphan proteins that the orphan signal sequence directs to the surface of a cell. Using these expression vectors and methods, an orphan protein can be cloned in the absence of a functional assay that detects such protein.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.