Patent · US Expired

Acarbose biosynthesis genes from actinoplanes sp., process for the isolation thereof and the use thereof

US5753501A · kind A · utility

2Cited by

3References

2Claims

0Family size

Assignee

BAYER HEALTHCARE LLC · DE

Inventors

Anneliese Crueger · Erkrath, DE
Wolfgang Piepersberg · Wuppertal, DE
Jurgen Distler · Berlin, DE
Ansgar Stratmann · Hattingen, DE

Key dates

Filing date	Feb 23, 1996
Grant date	May 19, 1998
Priority date	—
Expiry date	Feb 23, 2016

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC07K14/365
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

The invention relates to acarbose biosynthesis genes from actinomycetes, predominantly from Actinoplanes sp. SE 50/110 and its mutants, to a process for the isolation of acarbose biosynthesis genes from actinomycetes using a gene probe which has been derived from highly conserved protein regions of known dTDP-glucose dehydratase enzymes for finding the genes acbA (coding for dTDP-glucose synthase), acbB (coding for dTDP-glucose dehydratase) and acbC (coding for a cyclase, in part identical to AroB, bacterial 3-dehydroquinate synthases) or one or more acarbose biosynthesis genes from Actinoplanes sp., and to the use of the acarbose biosynthesis genes.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.