Quantitative immunochromatographic assays
US5753517A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Mar 29, 1996 |
| Grant date | May 19, 1998 |
| Priority date | — |
| Expiry date | Mar 29, 2016 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/54388
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Quantitative immunochromatographic assays for measuring the amount of an analyte, and an apparatus for use in the assays, are disclosed. The assays involve obtaining a fluid sample which contains the analyte; supplying a RAMP.TM. apparatus which includes a membrane having an application point, a detection zone, and a contact region, where the contact region is between the application point and the detection zone, and has antibody-coated particles imbedded within it; contacting the application point with the fluid sample; maintaining the RAMP.TM. apparatus under conditions sufficient to allow fluid to transport analyte by capillary action to the contact region, where the analyte binds to the antibody-coated particles; further maintaining the apparatus under conditions sufficient to allow fluid to transport analyte-bound antibody-coated particles to the detection zone, where they interact with a detection reagent; and detecting the amount of analyte-bound antibody-coated particles which have reacted with the detection reagent. Alternatively, the fluid sample can be contacted with the detection zone of the apparatus, and the antibody-coated particles are mobilized by addition of fluid…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.