Method for synthesizing soluble recombiant proteins from bacteria cells

US5756311A · kind A · utility

Assignee

• Max-Planck-Gesellschaft zur Föderung der Wissenschaften e.V. · US

Inventors

• Bernd Otto · Hannover, DE
• Gero Waschutza · Meinersen, DE
• Hayssam Zakaria · Hannover, DE

Key dates

• Filing date: Feb 10, 1997
• Grant date: May 26, 1998
• Expiry date: Feb 10, 2017

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12P21/02
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The invention relates to a method of manufacturing soluble recombinant proteins from bacteria, in which the plasmid contains the entire gene behind a promoter, this plasmid being expressed and the protein obtained being isolated and purified, in which the cell culture is raised to an OD 600 nm of 0.4 to 0.6; the cell culture is cooled over a period of 15 to 40 minutes to 20.degree. to 26.degree. C.; synthesis is induced by addition of 10 to 55 .mu.M of an inductor; and the induction phase is carried out at a temperature of 20.degree. to 26.degree. C. over a period of 3.5 to 6.5 hours.