Pokeweed antiviral protein mutants
US5756322A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Jul 11, 1995 |
| Grant date | May 26, 1998 |
| Priority date | — |
| Expiry date | Jul 11, 2015 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/8283
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Disclosed are PAP mutants having reduced phytotoxicity compared to wild-type PAP, and which retain wild-type PAP biological activity in plants. One group of PAP mutants is characterized by at least one amino acid substitution in the N-terminus of mature PAP, such as the Glycine 75 residue or the Glutamic acid 97 residue. Another group of preferred PAP mutants is characterized by mutations such as truncations in the C-terminal region of mature PAP. PAP mutants having from at least about 26 to about 76 mature PAP amino acids (not counting the 29-amino acid C-terminal extension of wild-type PAP) exhibit reduced phytotoxicity and retain PAP biological activity in plants. The disclosed PAP mutants may include the 22-amino acid N-terminal signal sequence and/or the C-terminal extension of wild-type PAP. Also disclosed are DNA molecules encoding the PAP mutants. The DNAs can be operably linked to a promoter functional in given host cells such as plants, and stably transformed into a vector functional in said cells. Procaryotic or eucaryotic hosts, e.g., yeast or plants, stably transformed with a mutant PAP-encoding DNA are further disclosed, as well as protoplasts stably transformed with …
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.