Detection of nucleic acids in cells by thermophilic strand displacement amplification
US5756702A · kind A · utility
26Cited by
2References
4Claims
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Key dates
| Filing date | Jan 10, 1997 |
| Grant date | May 26, 1998 |
| Priority date | — |
| Expiry date | Jan 10, 2017 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6881
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Thermophilic Strand Displacement Amplification (tSDA) for amplification of nucleic acid target sequences in situ in cells in suspension, on slides or in tissues is described. Excellent specimen morphology is preserved, and either DNA targets, RNA targets, or both may be selectively amplified. In situ amplification by tSDA is compatible with immunochemical techniques, so that both amplification of target sequences and immunological staining can be perfonned on the same specimen.
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