Sensitive nucleic acid sandwich hybridization assay

US5759773A · kind A · utility

Assignee

• The Public Health Research Institute Of the City of New York, Inc. · US

Inventors

• Sanjay Tyagi · New York, US
• Fred R. Kramer · Yonkers, US
• Paul M. Lizardi · San Antonio, US
• Ulf Landegren · Uppsala, SE
• Herman Blok · Wemeldinge, NL

Key dates

• Filing date: Feb 24, 1995
• Grant date: Jun 2, 1998
• Expiry date: Feb 24, 2015

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6862
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Nucleic acid sandwich hybridization assays are provided that incorporate one or a combination of background reduction steps. Those steps include use of a separate capture probe and separation from immobilized capture probes by cleavage and isolation. A very sensitive assay for RNA targets includes both of those steps, plus RNA binary probes, an RNA-directed RNA ligase and amplification by an RNA-directed RNA polymerase. Kits of reagents for performing assays according to this invention are also provided.