Polynucleotide-array assay and methods
US5759779A · kind A · utility
52Cited by
10References
7Claims
0Family size
Inventor
Key dates
| Filing date | Jan 11, 1996 |
| Grant date | Jun 2, 1998 |
| Priority date | — |
| Expiry date | Jan 11, 2016 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2035/00237
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method of analyzing the sequence of a polynucleotide analyte. The method includes contacting the analyte with a position-addressable array of oligonucleotides, each anchored to a solid support and having a 5'proximal and 3'-distal orientation. The hybridized oligonucleotides are then extended by strand-directed polymerase, to produce labeled, extended oligonucleotides at positions of the array corresponding to sequence matches between the array oligonucleotides and analyte regions. The pattern of label in the array is used to analyze analyte sequence.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.