Process for producing cDNA

US5759820A · kind A · utility

Assignee

• Dynal AS · NO

Inventors

• Erik Hornes · Oslo, NO
• Lars Korsnes · Oslo, NO

Key dates

• Filing date: Jul 25, 1994
• Grant date: Jun 2, 1998
• Expiry date: Jul 25, 2014

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10T436/196666
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The process includes the steps of: a) contacting a liquid containing mRNA with an insoluble support having DNA probes attached thereto via the 5' -terminus thereof whereby the MRNA is hybridized to said probes and hence to said support; b) removing said liquid; and c) adding enzymes and nucleotides in solution whereby the probe functions as a primer to produce single stranded cDNA on the mRNA probe. The probe DNA may be oligo-dT or a specific DNA sequence, such as one that is complementary to a conserved region in a class of mRNA molecules. The insoluble support comprises magnetic particles which are monodisperse polymer particles comprising superparamagnetic iron oxide, a coating to reduce non-specific binding and a substituent for attaching an oligonucleotide.