Genes for butyrobetaine/crotonobetaine-l-carnitine metabolism and their use for the microbiological production of l-carnitine
US5759824A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jul 2, 1996 |
| Grant date | Jun 2, 1998 |
| Priority date | — |
| Expiry date | Jul 2, 2016 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/743
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
An isolated DNA fragment including the genes bcoC, bcoA/B, bcoD coding for the enzymes for L-carnitine biosynthesis in .gamma.-butyrobetaine/crotonobetaine metabolism and, additionally, including the gene bcoT coding for a potential transport protein in .gamma.-butyrobetaine/crotonobetaine metabolism. Also, any vector which includes an isolated DNA fragment including the genes bcoC, bcoA/B, bcoD coding for the enzymes for L-carnitine biosynthesis in .gamma.-butyrobetaine/crotonobetaine metabolism and, additionally, including the gene bcoT coding for a potential transport protein in .gamma.-butyrobetaine/crotonobetaine metabolism. Also, biotechnological process for the production of L-carnitine characterized in that crotonobetaine and/or .gamma.-butyrobetaine is fermented in the presence of a suitable carbon and nitrogen source using a microorgansim and L-carnitine is isolated.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.