Lipopolysaccharide binding protein and process for producing the same
US5760177A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Feb 23, 1995 |
| Grant date | Jun 2, 1998 |
| Priority date | — |
| Expiry date | Feb 23, 2015 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S530/82
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
The invention provides a lipopolysaccharide (LPS) binding protein isolated from horseshoe crab. The LPS binding protein is isolated by (i) extracting the hemocyte membrane fraction of horseshoe crab with a polyethylene glycol ether type nonionic surface active agent in the presence of Ca ions, (ii) combining the extract with immobilized LPS under conditions that permit the LPS binding protein to bind the immobilized LPS to produce an LPS-LPS binding protein complex, and (iii) harvesting the LPS binding protein released from the complex in the presence of a chelating agent. The isolated LPS binding protein has a molecular weight of about 27,000 daltons as determined by SDS polyacrylamide gel electrophoresis and is operative to bind a lipopolysaccharide endotoxin. Accordingly, the isolated LPS binding protein can be used for detecting endotoxin and/or removing endotoxin from an injectable medicine.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.