Simultaneous sequencing of tagged polynucleotides
US5763175A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Nov 17, 1995 |
| Grant date | Jun 9, 1998 |
| Priority date | — |
| Expiry date | Nov 17, 2015 |
Classification
- Technology area (CPC B)Performing Operations; Transporting
- CPC primaryB01J2219/00659
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention provides a method for sequencing each polynucleotide of a population by using an oligonucleotide tag assigned to each such polynucleotide for transfering sequence information to a tag complement located on a spatially addressable array of such complements. That is, a unique tag is attached to each polynucleotide of a population which can be copied and used to shuttle sequence information to its complement at a fixed position on an array of such complements. After a tag hybridizes with its complement, a signal is generated that is indicative of the transferred sequence information. Sequences of the tagged polynucleotides are determined by repeated cycles of information transfer and signal detection at the positions of the corresponding tag complements.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.