Bacterial donor cell useful in conjugation
US5763187A · kind A · utility
0Cited by
1References
24Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jan 17, 1997 |
| Grant date | Jun 9, 1998 |
| Priority date | — |
| Expiry date | Jan 17, 2017 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/75
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to a modified bacterial donor cell useful in conjugation, the cell containing i) a plasmid comprising a DNA construct encoding the polypeptide of interest, and at least one cis-acting DNA sequence required for the transfer of said plasmid by conjugation in the presence of a trans-acting mobilizing element, and ii) at least one DNA sequence encoding said trans-acting mobilizing element, wherein the donor cell has a reduced capacity, relative to a parent cell, of producing a bactericidal agent which kills or prevents growth in a population of recipient cells.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.