Assays using cross-linked polypeptide fragments of .beta.-galactosidase
US5763196A · kind A · utility
3Cited by
14References
20Claims
0Family size
Assignee
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Key dates
| Filing date | Jan 26, 1996 |
| Grant date | Jun 9, 1998 |
| Priority date | — |
| Expiry date | Jan 26, 2016 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/37
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Formation of an intramolecular cross-link in enzyme donor polypeptide fragments of .beta.-galactosidase, thereby forming a cyclic enzyme donor which is hindered from complementation with an enzyme acceptor fragment to form active of .beta.-galactosidase. The cyclic enzyme donor can be linearized by cleaving to restore complementation ability. Assays in which such cyclic enzyme donors are linearized by specific analytes are disclosed, as well as novel homobifunctional bis-maleimido cross-linking agents of the formula ##STR1## wherein R is hydroxy or acetate.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.