Screening assays for compounds
US5763198A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jul 22, 1994 |
| Grant date | Jun 9, 1998 |
| Priority date | — |
| Expiry date | Jul 22, 2014 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/9121
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The invention is directed to rapid and quantitative assay systems for screening test compounds for their ability to modulate tyrosine kinase or phosphatase activities involved in signal transduction by determining the tyrosine phosphorylation state of a protein substrate using an anti-phosphotyrosine antibody and an antibody specific for the protein substrate. These assays may be practiced in a whole cell or cell-free system. The assays can be used to identify test compounds for use in therapeutic applications to disease processes in which tyrosine kinase or phosphatase activity in a signal transduction pathway contributes to a pathological process.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.