Process for the production of primers and template bank therefor
US5763227A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Apr 29, 1996 |
| Grant date | Jun 9, 1998 |
| Priority date | — |
| Expiry date | Apr 29, 2016 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6853
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention relates to a process for the production of a primer for consecutive DNA sequencing, in which at least two shortmers are hybridised adjacent to one another using a template and are then ligated with one another to form the primer, the process being characterised in that PA1 (a) a template in the form of a single strand of nucleotides is used in which the number of nucleotides is at least 6 and is not greater than approximately twice the total number of nucleotides of all the shortmers used, and PA1 (b) the primer formed is then separated from the template and from unreacted shortmers and recovered. The invention relates also to banks (libraries) of templates for the mentioned process.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.